The film used sound effects from Hanna-Barbera, Warner Bros., Cartoon Trax Volume 1, The Premiere Edition Volume 1, 20th Century Fox, Universal Studios, Rocky and Bullwinkle and Friends, The General Series 6000, Series 4000, Animal Trax, Animation Collection, Skywalker Sound and The International.
- Advantage Audio Fall Sound?
- Cartoon Suction Sound
- Disney - SMALL BONK
- Elektra Records, Blizzard/Sound Ideas, WIND - SIBERIAN WIND, WEATHER
- H-B BOING, CARTOON - LITTLE BOING
- H-B PLINK, CARTOON - HIGH PLINK
- H-B SLIDE, CARTOON - LONG SLIDE WHISTLE DOWN
- H-B SNAP, CARTOON - POP AND BIG SNAP WITH BOING
- H-B SPIN, CARTOON - WACKY SPIN WITH DRUM
- H-B ZIP, CARTOON - QUICK WHISTLE ZIP IN, HIGH/Sound Ideas, ZIP, CARTOON - RICCO ZIP IN 01 (R & B)
- Hollywoodedge, Airy Bubbles Wwet Po CRT018601 (pop only)
- Hollywoodedge, Ascending Whistles CRT057901
- Hollywoodedge, Bang Whonk CRT032404
- Hollywoodedge, Bicycle Bell Rings Sm PE190701
- Hollywoodedge, Big Metal Clangthud CRT031902
- Hollywoodedge, Big Metal Clangthud CRT031903
- Hollywoodedge, Bottle Rocket 2 Picco PE098501
- Hollywoodedge, Boings For Impacts CRT016701
- Hollywoodedge, Bounce Boing Drum CRT016901/Sound Ideas, BOINK, CARTOON - TIMP DOINK (Heard once during the song "Think" as a toilet paper bounces off Principal Benjamin Krupp.)
- Hollywoodedge, Bounce Jews Harp CRT015802
- Hollywoodedge, Bounce Jews Harp CRT015806/Sound Ideas, HIT, CARTOON - FLAT BONK
- Hollywoodedge, Cartoon Raspberries TE021504
- Hollywoodedge, Cat Domestic Meow Ang AT010501 (Heard when a dog attacks a cat while it was trying to play with a bird.)
- Hollywoodedge, Cat Domestic Meow Ang AT010603
- Hollywoodedge, Cats Fighting Loud Sc PE917007/Cats Two Angry YowlsD PE022601 (5th yowl; heard once when Principal Krupp used a flamethrower to burn a cat, which is censored by a censor bar.)
- Hollywoodedge, Clock Goes Crazy CRT037201 (Boing only.)
- Hollywoodedge, Comedy Body Fall Msx CRT017301
- Hollywoodedge, Crowd Med Shock PE961301/Crowd Reaction Shock PE142501
- Hollywoodedge, Crash Metal Shatter PE110201
- Hollywoodedge, Crash Metal Shatter PE110401
- Hollywoodedge, Crash Train Car Mix PE111601
- Hollywoodedge, Cymbal Crash BoingQ CRT016405/Sound Ideas, COMEDY - BOING 04
- Hollywoodedge, Dog Small Yelps Barks PE023301
- Hollywoodedge, Dolphin Chirps Vocal PE024601/Dolphins Chirping TE015902
- Hollywoodedge, Doppler Car Horn By Ap PE077401/Doppler Horn By HighP CT053001
- Hollywoodedge, Doppler Horn By LeftT PE079801
- Hollywoodedge, Funny Multi Swishes CRT054102/Sound Ideas, SWISH, CARTOON - TWIRLING SWISH, MEDIUM
- Hollywoodedge, Funny Rapid GlassyM CRT041401
- Hollywoodedge, Funny Swish CRT053904
- Hollywoodedge, Giggling Two Childre PE131001/Two Young Kids Giggle PE143501
- Hollywoodedge, Hammer Hitting An Anv TE037903
- Hollywoodedge, Head Bonk CRT2016604
- Hollywoodedge, Head Bonk Wbell Drum CRT031901
- Hollywoodedge, Head Bonk Wcymbal Cr CRT033004
- Hollywoodedge, Head Bonk Whorn 3 Typ CRT032401
- Hollywoodedge, Heavy Bonk Wbass Dru CRT033001
- Hollywoodedge, Heavy Head Bonk Fall CRT033002
- Hollywoodedge, Honk With Cymbal Crash CRT033005
- Hollywoodedge, Hub Cap Falls On Aspha PE077701
- Hollywoodedge, Jews Harp 9 Various Je AC020101
- Hollywoodedge, Large Ceramic Pot Bre TE023001/Pottery Drop Break PE112401
- Hollywoodedge, Large Explosion With Lon PE280201
- Hollywoodedge, Lg Metal Wheel Creak CRT053701
- Hollywoodedge, Lightning 08 CloseG SIG012601
- Hollywoodedge, Lincoln Town Car In Sk PE073501
- Hollywoodedge, Low Pitched Squeak CRT049301
- Hollywoodedge, Long Creaky Stretch CRT049405
- Hollywoodedge, Long Stretch Pop CRT049308
- Hollywoodedge, Med High Pitch Boing CRT016001
- Hollywoodedge, Medium Crowd AdultsY PE142901
- Hollywoodedge, Medium Exterior Crow PE140401
- Hollywoodedge, Metal Bonk Wlowpitc CRT031703
- Hollywoodedge, Metal Impacts Low Lar FS012305
- Hollywoodedge, Old Car Horns Single CRT021302
- Hollywoodedge, Police Wailer Siren PE080801
- Hollywoodedge, Puma Cougar Hiss Seri AT017001 (Last snort; heard when a cat growls before jumping up and attacking a bird.)
- Hollywoodedge, Quick Whistle Zip By CRT057501
- Hollywoodedge, Quick Whistle Zip By CRT057503 (high pitched)
- Hollywoodedge, Quick Whistle Zip By CRT057504 (Heard twice at the end as Mr. Krupp throws his hair away during the transformation of Captain Underpants followed by Ascending Whistles CRT057901.)
- Hollywoodedge, Quick Whistle Zip By CRT057505
- Hollywoodedge, School Bell Long Ring PE193201
- Hollywoodedge, Several Rapid Swish CRT054101
- Hollywoodedge, Short Bulb Horn Honk CRT020701 (Heard when Captain Underpants falls on the treehouse with a thud in front George and Harold.)
- Hollywoodedge, Short Nose Blowsbul CRT021101
- Hollywoodedge, Single High Pit Beeps PE190201
- Hollywoodedge, Small Group Kids Chee PE142801/Children Sml Grp Yell PE954901 (Heard when the Jerome Horwitz Elementary students enter the art classroom that George and Harold unlock it.)
- Hollywoodedge, Springy Boinge Long CRT016202
- Hollywoodedge, Tel Ring Modern Merli PE597201
- Hollywoodedge, Thin Metal Clang CRT031701
- Hollywoodedge, Thin Metallic Pot Or CRT031202
- Hollywoodedge, Tiger Growl Sharp Thr AT019401
- Hollywoodedge, Twangy Boings 7 Type CRT015901
- Hollywoodedge, Wet Splats Various CRT052101 (5th splat; heard in the video projector as a pie has fallen onto a person's face.)
- Hollywoodedge, Whistle Wbulb Horn CRT020801
- Hollywoodedge, Wood Door 3 Open SlamM PE180301
- Hollywoodedge, Wubble Wobble Spring PE940704
- Jurassic Park, T-Rex - Attack Roar (Used for a bird.)
- MGM BITE, CARTOON - BIG CRUNCHY BITE
- SKYWALKER, BUBBLES - HEARTS BUBBLING, APPEARING
- SKYWALKER, BUBBLES - THICK, GLOPPY MUD BUBBLES
- SKYWALKER, DOINK - MR. D.N.A. DOINKING
- SKYWALKER, DOOR - LOCKER DOOR CLOSING
- SKYWALKER, DOOR - LOCKER DOOR OPENING
- SKYWALKER, FOOTSTEP - ANDY FOOTSTEPS
- SKYWALKER, FOOTSTEP - OMNIDROID FOOTSTEPS
- SKYWALKER, HIT - UNDERPANTS HIT (Debut)
- SKYWALKER, METAL - ROBOT PROBE'S EYE CLOSE, LID
- SKYWALKER, METAL - TRANSFORMER CLANGING
- SKYWALKER, ROAR - GIANT BIRD ROAR (Debut)
- SKYWALKER, ROLLER SKATES - TALLULAH'S ROLLER SKATING
- SKYWALKER, SCI-FI GUN - PROFESSOR POPPYPANT'S SIZEIRATOR 2000 BLAST (Debut)
- SKYWALKER, SCI-FI - ROBOT PROBE'S EYE SENSOR
- SKYWALKER, SLINGSHOT - UNDERPANTS SLINGSHOT (Debut)
- SKYWALKER, SQUISH - B.O.B. SQUISHING
- SKYWALKER, STRETCH - MR. D.N.A. STRETCHING AND SNAP
- SKYWALKER, WHOOSH - SINGLE SWISH
- Sound Ideas, ALARM, FIRE - FIRE BELL: RINGING
- Sound Ideas, BELL, COWBELL - COWBELL RATTLE, LONG
- Sound Ideas, BIRD, HAWK - SINGLE HAWK SCREECHING, ANIMAL
- Sound Ideas, BITE, CARTOON - BIG CHOMP (Heard once when a beartrap flies in and clamps onto Principal Krupp's face.)
- Sound Ideas, BITE, CARTOON - BONE BITE (Heard faintly when a dog bites Captain Underpants' cape.)
- Sound Ideas, BLINK, CARTOON - XYLO EYE BLINKS
- Sound Ideas, BOING, CARTOON - BIG, LONG BOING
- Sound Ideas, BOING, CARTOON - FLAT JEWS HARP BOING
- Sound Ideas, BOING, CARTOON - GONG BOING
- Sound Ideas, BOING, CARTOON - RIPPLE BOING (reversed)
- Sound Ideas, BOING, CARTOON - SMALL BOING
- Sound Ideas, BOING, CARTOON - SPROING 01
- Sound Ideas, BOING, CARTOON - THIN WOOD BOING
- Sound Ideas, BOINK, CARTOON - BOINK 01
- Sound Ideas, BOINK, CARTOON - DOINK 01
- Sound Ideas, BOINK, CARTOON - DOINK 03
- Sound Ideas, BOINK, CARTOON - HOYT'S BOINK
- Sound Ideas, BOMB, WHISTLE - BOMB FALLING: WHISTLE-BY 01
- Sound Ideas, BORK, CARTOON - LOW BORK
- Sound Ideas, CARTOON, ACCENT - WHIZ, BONKS
- Sound Ideas, CARTOON, AIRPLANE - PROP PLANE PASS BY FAST
- Sound Ideas, CARTOON, BIRD - HEAD BONK BIRDS CHIRPING
- Sound Ideas, CARTOON, BOING - LOW TIMPANI, WOBBLE, PERCUSSION, MUSIC, DRUMS (Heard when Captain Underpants falls onto a gorilla-shaped balloon.)
- Sound Ideas, CARTOON, BOING - STRING PLUCK
- Sound Ideas, CARTOON, BOING - THIN METAL PING AND WARBLE 01
- Sound Ideas, CARTOON, HORN - SHORT HORN TOOT, MEDIUM
- Sound Ideas, CARTOON, POP - CORK SQUEAK AND POP
- Sound Ideas, CARTOON, SNAP - THIN RUBBER SNAP, TWO TIMES (2nd snap)
- Sound Ideas, CARTOON, SQUAWK - BIG HORN SQUAWK/SQUAWK, CARTOON - BIG HORN SQUAWK
- Sound Ideas, CARTOON, SQUAWK - SMALL HORN BONK 01
- Sound Ideas, CARTOON, SQUEAK - SEVERAL RUBBER SQUEAKS, STRETCH
- Sound Ideas, CARTOON, SWISH - SINGLE FAST WHIPPY SWISH BY
- Sound Ideas, CARTOON, SWISH - WHIPPY VOCAL SWISH
- Sound Ideas, CARTOON, TWANG - WOBBLING TWANG
- Sound Ideas, CARTOON, WARBLE - WARBLE SHEET
- Sound Ideas, CARTOON, WHISTLE - FLUTTER WHISTLE: LONG SPINNING WHISTLE
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST SLIDE UP, ZIP 02
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST ZIP
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST ZIP DOWN
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST ZIP UP
- Sound Ideas, CARTOON, WHIZZ - FAST WHIZZ BY
- Sound Ideas, CARTOON, WINDOW - ROLLER BLIND SNAP UP AND RATTLE
- Sound Ideas, CARTOON, ZIP - MEDIUM 01
- Sound Ideas, CARTOON, ZIP - QUICK ZIP IN 01
- Sound Ideas, CAT - DOMESTIC: SINGLE MEOW, ANIMAL 02
- Sound Ideas, CAT - DOMESTIC: SINGLE MEOW, ANIMAL 03
- Sound Ideas, CHILDREN - CHEERING, CROWD 02
- Sound Ideas, CHILDREN, CROWD - SMALL STUDIO AUDIENCE OF CHILDREN: APPLAUSE AND CHEER, CHEERING 02
- Sound Ideas, CRASH, CARTOON - KEN'S BASS, DRUM AND CYMBAL CRASH, MUSIC, PERCUSSION
- Sound Ideas, CRASH, CARTOON - SHARP BASS, DRUM AND CYMBAL CRASH, MUSIC, PERCUSSION
- Sound Ideas, CRASH, CARTOON - TONY'S BASS, DRUM AND CYMBAL CRASH, MUSIC, PERCUSSION
- Sound Ideas, ELECTRONIC - MAGICAL SWISH 03
- Sound Ideas, GUN, RICOCHET - SHORT THIN RICOCHET, BULLET 02
- Sound Ideas, HIT, CARTOON - BIG HEAD BONK
- Sound Ideas, HIT, CARTOON - BRAKE DRUM AND BULB HORN HIT (Heard when a car crashes right into Captain Underpants followed by Hollywoodedge, Cat Domestic Meow Ang AT010603.)
- Sound Ideas, HIT, CARTOON - COCONUT HIT 01
- Sound Ideas, HIT, CARTOON - COCONUT HIT 02
- Sound Ideas, HIT, CARTOON - HEAVY TIMP RISE
- Sound Ideas, HIT, CARTOON - HOLLOW METALLIC HEAD KONK AND HORN
- Sound Ideas, HIT, CARTOON - KABONG
- Sound Ideas, HIT, CARTOON - SOLID TIMP RISE
- Sound Ideas, HIT, CARTOON - ZIP AND HEAVY THUMP (Heard once when Captain Underpants crashes into the school bus before he drives.)
- Sound Ideas, HIT, HUMAN - BIG HEAVY FACE SLAP, CARTOON 01
- Sound Ideas, HIT, METAL - ANVIL FALL ON HEAD, CARTOON 01
- Sound Ideas, HORN, CARTOON - OLD BULB HORN
- Sound Ideas, HORSE - INTERIOR: WHINNY, ANIMAL 02
- Sound Ideas, PLUCK, CARTOON - VAROOP
- Sound Ideas, RICOCHET - CARTOON RICCO 01/ZIP, CARTOON - RICCO ZIP OUT 01
- Sound Ideas, RICOCHET - CARTOON RICCO 07 (Cut-short; heard when Principal Krupp throws away his hair during the transformation of Captain Underpants.)
- Sound Ideas, RICOCHET - SLICK RICCO 01/GUN, RICOCHET - SINGLE RICOCHET, BULLET 01
- Sound Ideas, RICOCHET - SLICK RICCO 02/GUN, RICOCHET - SINGLE RICOCHET, BULLET 05
- Sound Ideas, RUN, CARTOON - BLOP GALLOP, LONG/SHORT
- Sound Ideas, RUN, CARTOON - FAST RUN, LONG/SHORT
- Sound Ideas, RUN, CARTOON - TEMPLE BLOCK RIOT, LONG/MEDIUM/SHORT
- Sound Ideas, RUN, CARTOON - WIND WHISTLE SCAT
- Sound Ideas, SIREN - 'WAIL' SIREN, POLICE, AMBULANCE, FIRE TRUCK 01
- Sound Ideas, SKID, CARTOON - BROKEN SKID
- Sound Ideas, SLIDE, CARTOON - FIDDLE SLIDE DOWN 01
- Sound Ideas, SLIDE, CARTOON - FIDDLE SLIDE DOWN, SHORT (high pitched)
- Sound Ideas, SLIDE, CARTOON - FIDDLE SLIDE UP 01 (high pitched)
- Sound Ideas, SLIDE, CARTOON - FIDDLE SLIDE UP, SHORT 02 (high pitched)
- Sound Ideas, SPIN, CARTOON - SPIN AND BOING
- Sound Ideas, SPIN, CARTOON - SPIN COMMOTION
- Sound Ideas, SQUEAK, WHEEL - SMALL SQUEAKY WHEEL TURNING, CARTOON
- Sound Ideas, SQUISH, CARTOON - LITTLE SQUISH
- Sound Ideas, SQUISH, MUD - SINGLE MUD BUBBLE SQUISH 02
- Sound Ideas, STEAM - HEAVY STEAM RELEASE
- Sound Ideas, STRETCH, CARTOON - RUBBER STRETCH, LONG
- Sound Ideas, SWISH, CARTOON - FAST TWIRLING SWISH, LONG
- Sound Ideas, TEETH, CARTOON - SCOOBY'S TEETH CHATTER, LONG/SHORT
- Sound Ideas, THUMP, CARTOON - TUBE THUNK 01
- Sound Ideas, THUNDER - BIG THUNDER CRACK AND RUMBLE, WEATHER
- Sound Ideas, TRAIN, STEAM - STEAM TRAIN, LOCOMOTIVE, HORN, WHISTLE, DIGIFFECTS (Heard once in a higher pitch.)
- Sound Ideas, TWANG, CARTOON - BOWANG
- Sound Ideas, TWANG, CARTOON - HOYT'S BOW TWANG (Heard once along with Sound Ideas, BOINK, CARTOON - DOINK 03, when Captain Underpants bounces on a trampoline before flying off.)
- Sound Ideas, TWANG, CARTOON - METALLIC RULER VIBRATE
- Sound Ideas, WHINE, CARTOON - SHELL SCREAMING WHINE DOWN (Heard quadrice:)
- When a pile of water balloons fall on Principal Krupp.
- When Captain Underpants is about to fall onto the bank robbers after George and Harold burst an ape-shaped balloon.
- When Turbo Toilet 2000 throws the house away.
- When Captain Underpants falls behind the Jerome Horwitz Elementary students after his cape rips.
- Sound Ideas, WHISTLE, STEAM - FACTORY WHISTLE, LARGE FACTORY
- Sound Ideas, WHISTLE, STEAM - FLINTSTONE FACTORY WHISTLE, LONG/SHORT, CARTOON
- Sound Ideas, WOLF - LONG HOWL, ANIMAL 01
- Sound Ideas, ZIP, CARTOON - BIG WHISTLE ZING OUT (Heard once in a low volume when Professor Poopypants first yells at George and Harold for asking him about why he wants to get rid of laughter.)
- Sound Ideas, ZIP, CARTOON - KEEN ZIP OUT
- Sound Ideas, ZIP, CARTOON - QUICK WHISTLE ZIP OUT (Heard when Captain Underpants is being blown by the Turbo-Toilet 2000. This is the fourth DreamWorks Animation film to use the sound effect after Madagascar, Kung Fu Panda and Madagascar 3: Europe's Most Wanted.)
- Sound Ideas, ZIP, CARTOON - QUICK WHISTLE ZIP OUT, HIGH (Heard once when Principal Krupp throws a newspaper as he is shocked that George and Harold are reunited.)
- WB CARTOON, BOING - WACKY JEWS HARP BOING
- WB CARTOON, HEAD SHAKE - TROMBONE GOBBLE
- Wilhelm Scream