The game mainly uses Series 6000 and Warner Bros. sound effects, as well as selections from the Series 1000 and Universal Studios libraries. One sound effect from the Lucasfilm Sound Effects Library can also be heard in-game.
- Best Service, HUMAN, YELL - YELL: FEMALE (Edited versions of the sound are used as death cries for Goombas, Koopas, and Fly Guys. Also used as a voice clip for Koopa the Quick.)
- Hollywoodedge, Chimpanzee Screeche PE026201
- Robert Hall, Throwing Pebbles in Water/Sound Ideas, WATER, SPLASH - PEBBLES INTO STILL WATER (2nd half of 1st splash)
- King Bob-omb Sound
- SM64 1-Up Sound/Extra Life Sound
- SM64 Big Stomp Sound (Debut; used for Bowser; a high-pitched version is used for impacts and big enemies walking like Whomps; edited versions of this sound is used for boulders and a Grindel jumping.)
- SM64 Bob-omb Sound
- SM64 Boss Death Sound
- SM64 Bounce Sound
- SM64 Burst Sound
- SM64 Camera Sound 1
- SM64 Camera Sound 2
- SM64 Camera Sound 3
- SM64 Chain Sound (Used for Chain Chomp.)
- SM64 Chomp Sound
- SM64 Coin Drop Sound
- SM64 Coin Sound
- SM64 Correct Sound
- SM64 Cursor Sound 1
- SM64 Cursor Sound 2
- SM64 Enemy Death Sound 1 (Edited versions of the sound are used for Boos and Mr. I's.)
- SM64 Enemy Death Sound 2
- SM64 Enemy Death Sound 3
- SM64 Enemy Death Sound 4 (Used for Wiggler and Piranha Plants.)
- SM64 Enemy Death Sound 5 (Used for Chuckya.)
- SM64 Enemy Hit Sound (Used for Wiggler.)
- SM64 Enemy Jump Sound 1
- SM64 Enemy Jump Sound 2
- SM64 Error Sound
- SM64 Exit Sound
- SM64 Game Over Sound
- SM64 Ground Pound Sound
- SM64 Health Alert Sound
- SM64 Health Meter Sound
- SM64 Heave-Ho Sound
- SM64 High Score Sound
- SM64 Impact Sound
- SM64 Interact Sound
- SM64 Key Drop Sound
- SM64 Lakitu Sound
- SM64 Mad Piano Sound
- SM64 Pause Sound
- SM64 Penguin Sound (debut; a low-pitched version is used for Mama Penguin.)
- SM64 Pipe Appear Sound
- SM64 Pipe Disappear Sound
- SM64 Piranha Plant Chomp Sound
- SM64 Projectile Sound 1
- SM64 Projectile Sound 2
- SM64 Puzzle Tile Sound (Used for the puzzle tiles sliding in Lethal Lava Land.)
- SM64 Rabbit Hop Sound (Used for MIPS.)
- SM64 Red Coin Sound
- SM64 Select Sound
- SM64 Slide 1
- SM64 Spinning Heart Sound
- SM64 Star Appear Sound
- SM64 Star Sparkle Sound
- SM64 Stomp Sound 1
- SM64 Stomp Sound 2
- SM64 Text Sound 1
- SM64 Text Sound 2
- SM64 Text Sound 3
- SM64 Thwomp Grunt
- SM64 Transition Sound
- SM64 Walk Sound 1 (Used for Goombas.)
- SM64 Walk Sound 2 (Used for Bob-ombs.)
- SM64 Walk Sound 3 (Used for Skeeters.)
- SM64 Warp Sound (An edited version is used for Moneybags; a low-pitched version is used for Bowser teleporting.)
- SM64 Whomp King Sound (debut; used for Whomp King and Eyerok; a high-pitched and sped-up version is used for Bullies; a lower pitch and sped-up version is used for Big Bullies and a Chill Bully.)
- SM64 Wiggler Sound
- SM64 Wobble Sound
- SM64 Zip Sound
- SMW Yoshi Sound
- Sound Ideas, ALARM, CAR - SET CAR ALARM, AUTO (Used for penguin footsteps.)
- Sound Ideas, ANIMAL, CREATURE - LARGE ANIMAL DEATH SCREAM (An edited version is used when Bowser is defeated.)
- Sound Ideas, ANIMAL, CREATURE - LARGE ANIMAL GROWL 04 (An edited version of the 2nd half of the sound is used whenever Bowser breathes fire.)
- Sound Ideas, ANVIL - SINGLE HIT ON ANVIL WITH HAMMER, METAL 02 (Used in a low pitch when bumping into a Bully.)
- Sound Ideas, BIRD, BUDGIES - AMBIENCE: VARIOUS CALLS (The 1st call is used in a low pitch for ambience outside Peach's Castle.)
- Sound Ideas, BIRD, CANARIES - AMBIENCE: VARIOUS CALLS, CANARY (A small portion of the sound is used in a very low pitch for ambience outside Peach's Castle.)
- Sound Ideas, BIRD, GOOSE - BUFF GOOSE: CALLING, GEESE, ANIMAL 01 (The last call is used for Klepto.)
- Sound Ideas, BIRD, HAWK - CALLS, ANIMAL (A small portion is used for Swoops.)
- Sound Ideas, BIRD, NIGHTINGALE - SINGING, ANIMAL (A small portion of the sound is used for ambience outside Peach's Castle.)
- Sound Ideas, BIRD, SKYLARK - SINGING, ANIMAL (Unused, but can be found in the game's files. Note that game's remake makes use of this sound.)
- Sound Ideas, BUBBLES, UNDERWATER - STEADY UNDERWATER BUBBLES (A small portion is used when swimming.)
- Sound Ideas, CAMERA - 35 MM SLR WITH AUTO WINDER: SINGLE SHOT (The 1st portion of the sound is used when changing camera modes.)
- Sound Ideas, CARTOON, BREATHE - BIG INHALE THROUGH MOUTH (Used in a low pitch when Bowser is about to breath fire.)
- Sound Ideas, CARTOON, ELECTRICITY - SHORT ELECTRICAL ARC (A small portion is used for Amps.)
- Sound Ideas, CARTOON, FUSE - LONG FUSE SIZZLING, SIZZLE (Used for Bob-ombs' fuses.)
- Sound Ideas, CARTOON, HIT - METALLIC SMACK (An edited version is used for Metal Mario's footsteps.)
- Sound Ideas, CARTOON, POP - WHOOSHY POP
- Sound Ideas, COMEDY - BOING 02 (Used when breaking blocks.)
- Sound Ideas, COMEDY - BOING 04 (Used when a Crazed Crate bounces after being grabbed.)
- Sound Ideas, COMEDY - BOING 05 (Seems to be unused.)
- Sound Ideas, COUGAR - GROWL, ANIMAL, MOUNTAIN LION, CAT 01 (A high pitched version is used as Bowser's roar.)
- Sound Ideas, CREAK, DOOR - SHORT DOOR CREAK 03 (Used in a very low pitch.)
- Sound Ideas, DOG, ROTTWEILER - MEDIUM DOG, BARKING, ANIMAL (Heard in a low pitch for the Chain Chomp.)
- Sound Ideas, DOOR, DUNGEON - DUNGEON DOOR: LIGHT CLOSE AND LATCH, CASTLE (Only part of the sound is used.)
- Sound Ideas, DOOR, WOOD - CLOSE 04
- Sound Ideas, DOOR, WOOD - OPEN 04
- Sound Ideas, ELECTRONIC - MAGICAL ZAP 06 (A low pitched and edited version is used when exiting paintings.)
- Sound Ideas, ELECTRONIC - MAGICAL ZIP 01 (Used when entering mini-courses.)
- Sound Ideas, EXPLOSION - LARGE EXPLOSION 06/07/08 (Used in various pitches.)
- Sound Ideas, FOOTSTEPS, SAND, DRY - MALE BAREFOOT: WALK (3rd footstep)
- Sound Ideas, GUN, ANTI TANK - 20MM: SINGLE SHOT 01 (Used as a starting gun whenever a race begins.)
- Sound Ideas, GUN, TANK - HOWITZER: SINGLE SHOT 03 (Used for cannons.)
- Sound Ideas, HIT, CLOTH - HIT INTO CLOTH COVERED OBJECT 01 (Seems to be unused.)
- Sound Ideas, HIT, METAL - LARGE HEAVY SHEET METAL HIT, CARTOON 04 (Used in a very low pitch.)
- Sound Ideas, HUMAN, LAUGH - COMICAL LAUGH: MALE (This game also uses the sound in a very low pitched and slowed down version for Bowser's laugh.)
- Sound Ideas, HUMAN, SIGH - SIGH: FEMALE 01 (An edited version is used when a Piranha Plant is defeated.)
- Sound Ideas, INDUSTRY, HYDRAULIC - ELECTRIC HYDRAULIC MACHINE: UP FULL, FACTORY (Used for cannons.)
- Sound Ideas, MONKEY - VOCALIZING, ANIMAL, APE 01 (The scream at 0:38.525 is used in a normal pitch for Ukikis.)
- Sound Ideas, MOTOR, CARTOON - JALOPY GIZMO MOTOR: START, RUN, STOP 01 (The first portion of the sound is used in a low pitch for various things, such as the clock on the castle's 3rd floor.)
- Sound Ideas, MOTOR, ELECTRIC - ELECTRIC INDUSTRIAL MOTOR 03 (Used in various pitches.)
- Sound Ideas, SIZZLE - FINE GRANULAR SIZZLING, CARTOON
- Sound Ideas, TANK, MILITARY - SHERMAN TANK: GUN BREECH: OPEN (Used for cannons.)
- Sound Ideas, TOMBSTONE - STONE DRAGGING ON STONE, GRIND 11
- Sound Ideas, VOLCANO - VOLCANO: INTERIOR, LAVA GURGLING, BUBBLING 01 (Used for ambience inside the volcano in Lethal Lava Land.)
- Sound Ideas, WATER, BUBBLES - SWIRLING BUBBLES
- Sound Ideas, WATER, SPLASH - LARGER, BIG THUMP & BIG WAVE (The 4th splash is used when the player makes Mario jump into water.)
- Sound Ideas, WINCH - CHAIN PULLED BY WINCH 02 (Used when aiming a cannon.)
- Sound Ideas, WIND - HOWLING HORROR WIND, WEATHER
- WB CARTOON, SWISH - SINGLE WHIP SWISH 04 (Used when doing a midair flip.)
Beta
NOTE: These sounds were only used in the game's beta, and were not used in the final game. Mario's voice clips in the beta are various male and female voice samples taken from the Voice Spectral Volume 1 library, with the exception of the burning/falling scream being a recording of Mel Blanc's voice from the Warner Bros. Sound Effects Library. The general consensus is that they were placeholder sounds for Mario, before Charles Martinet's recorded his own voice clips.
- Hollywoodedge, Puma Cougar Roar Clas AT016801/COUGAR - GROWL, ANIMAL, MOUNTAIN LION, CAT 03 (Used for Bowser.)
- HUMAN, VOCAL - “HAUGH!”: MALE
- HUMAN, VOCAL - “HOO!”: FEMALE
- HUMAN, VOCAL - “HUAH!”: MALE
- HUMAN, VOCAL - “NO!”: FEMALE
- HUMAN, VOCAL - “OU!”: MALE
- HUMAN, VOCAL - “SHORT HOO!”: FEMALE
- HUMAN, VOCAL - “YAHAA!”: MALE
- HUMAN, VOCAL - “YEAH!”: FEMALE
- SM64 Beta Coin Sound
- Sound Ideas, CARTOON - BIG HIT AND PAINFUL YELL
- Sound Ideas, CARTOON, YELL - PAINFUL YELL 01
- Sound Ideas, DUCK, CARTOON - QUICK QUACK
- Sound Ideas, HUMAN, GROAN - GROAN AND GRUNT: MALE 02 (3rd groan only, higher pitched)