The film used sound effects from Hanna-Barbera, Warner Bros., Cartoon Trax Volume 1 + 2, The Premiere Edition Volume 1 + 2 + 5, The General Series 6000, Skywalker Sound, Series 4000 and The Super Single Volume 1.
- H-B BLINK, CARTOON - HIGH EYE BLINKS
- H-B ZIP, CARTOON - QUICK ZIP
- Hollywoodedge, Bang Whonk CRT032404
- Hollywoodedge, Basketball Bounces PE304201 (Heard in the Sony Pictures Animation logo.)
- Hollywoodedge, Belch 7 Long Disgusti PE138601 (Used in a low pitch for a glow bunny.)
- Hollywoodedge, Big Metal Clangthud CRT031903
- Hollywoodedge, Bird Seagull Four Sin PE021601
- Hollywoodedge, Bounce Boing Drum CRT016901/Sound Ideas, BOINK, CARTOON - TIMP DOINK
- Hollywoodedge, Cartoon Boing Jews Ha PE163801
- Hollywoodedge, Cartoon Streaks 6 SS016506
- Hollywoodedge, Cat Domestic Meow Ang AT010603
- Hollywoodedge, Catfssst Yowl Hiss CRT012603
- Hollywoodedge, Cats Fighting Loud Sc PE917007/Cats Two Angry YowlsD PE022601
- Hollywoodedge, Drinking Glass Smash PE583201/Small Shatter SmallP PE284501
- Hollywoodedge, Funny Sm Creatur Vox CRT013201
- Hollywoodedge, Funny Swish CRT053904
- Hollywoodedge, Gusts Heavy Cold Wind PE031601/Whistling Wind Stead PE033301
- Hollywoodedge, Head Bonk CRT2016604
- Hollywoodedge, Head Bonk Wbell Drum CRT031901
- Hollywoodedge, High Pitch Moose Cal CRT013101
- Hollywoodedge, Honk With Cymbal Crash CRT033005
- Hollywoodedge, Low Pitched Squeak CRT049301
- Hollywoodedge, Rapid High Percuss CRT041408
- Hollywoodedge, Screams 1 Woman Singl PE133501
- Hollywoodedge, Short Bass Honk WCow CRT032406
- Hollywoodedge, Toilet Flush 2 Old Typ PE167401
- Hollywoodedge, Waterfall Large Roar PE044401
- Hollywoodedge, Whistle Human Finger PE139801
- SKYWALKER, AMBIENCE - BEACH AMBIENCE
- SKYWALKER, AMBIENCE - FORBIDDEN FOREST AMBIENCE (debut)
- SKYWALKER, ANIMAL - CRABS CHITTERING (Debut; used for the crabs.)
- SKYWALKER, ANIMAL - HORSE NEIGHING (Used in a higher pitch as one of the sounds for glow bunnies.)
- SKYWALKER, ANIMAL - HORSE WHINNYING (Used in a higher pitch as one of the sounds for glow bunnies.)
- SKYWALKER, ANIMAL - RABBITS CHITTERING (Debut; used for the rabbits.)
- SKYWALKER, ANIMAL - RACCOON VOCALIZING (Used for the dragonflies.)
- SKYWALKER, ANT - PRINCESS ATTA AND DOT'S FLAPPING WINGS
- SKYWALKER, BIRD - JACQUIMO'S FLAPPING WINGS
- SKYWALKER, BIRD - MONTY CHIRP (debut)
- SKYWALKER, BIRD - MONTY'S FLAPPING WINGS (debut)
- SKYWALKER, BIRD - MONTY SCREECH (debut)/SKYWALKER, ROAR - KOMODO RHINO ROAR (low pitched)
- SKYWALKER, BIRD - MONTY SQUAWKING (debut)
- SKYWALKER, BOW - ARROW LAUNCH FIRE
- SKYWALKER DOOR BANGING SOUND
- SKYWALKER DOOR CLOSING SOUND
- SKYWALKER DOOR CREAKING SOUND
- SKYWALKER DOOR OPENING SOUND
- SKYWALKER DOOR SLAMMING SOUND
- SKYWALKER, DOOR - WOODEN DOOR; OPEN & CLOSE; CREAKING; VARIOUS
- SKYWALKER, DRAGON - FIRE BREATHING (Used for the dragonflies when they breathe fire.)
- SKYWALKER EXPLOSION 01
- SKYWALKER, EXPLOSION - CAVE EXPLOSION (debut)
- SKYWALKER, EXPLOSION - PAC-MAN CHOMPING
- SKYWALKER, HUMAN - FEMALE GRUNT (debut)
- SKYWALKER, HUMAN - SMURFBERRIES, BITE, CHOMP, CHEWING (debut)
- SKYWALKER, INSECT - DRAGONFLY WINGS FLAPPING (debut)
- SKYWALKER, INSECT - FIREFLIES CHITTERING (debut)
- SKYWALKER, MACHINE - GARGAMEL'S MACHINE (debut)
- SKYWALKER, MOVEMENT - CRAB MOVEMENTS
- SKYWALKER, POP - SMALL SUCTION POPS
- SKYWALKER PUNCHING SOUNDS
- SKYWALKER, ROAR - CYBUG SCREAM
- SKYWALKER, ROLLER SKATES - TALLULAH'S ROLLER SKATING
- SKYWALKER, RUSTLE - BUSH RUSTLING
- SKYWALKER, SEQUENCE - FREEZE BALL SEQUENCE (debut)
- SKYWALKER, SQUISH - B.O.B. SQUISHING
- SKYWALKER. TOY - CHECKERS BOARD GAME PINS RATTLING
- SKYWALKER, TOY - LENNY FOOTSTEPS
- SKYWALKER, WATER - SLOSHES AND LAPPING, VARIOUS
- Sony Ding (Heard in the Sony logo from 2014.)
- Sound Ideas, BELL, FIGHT - BOXING RING FIGHT BELL: SINGLE RING, SPORTS
- Sound Ideas, BLINK, CARTOON - XYLO EYE BLINKS
- Sound Ideas, BOING, CARTOON - BIG, LONG BOING
- Sound Ideas, BOING, CARTOON - HOYT'S BOING (Heard when Monty lands right into the wall of Gargamel's lair.)
- Sound Ideas, BOING, CARTOON - SPROING 01
- Sound Ideas, CARTOON - BIG STRETCH AND SNAP, SLINGSHOT
- Sound Ideas, CARTOON - HARD HEAD BONK WITH TWITTERING BIRDS, HIT
- Sound Ideas, CARTOON, BIRD - HEAD BONK BIRDS CHIRPING
- Sound Ideas, CARTOON, HIT - ELEPHANT PUNCH
- Sound Ideas, CARTOON, HIT - METALLIC SMACK
- Sound Ideas, CARTOON, HIT - SINGLE TIMPANI HIT AND RISE
- Sound Ideas, CARTOON, HORN - SHORT HORN TOOT, MEDIUM
- Sound Ideas, CARTOON, POP - BIG CORK POP
- Sound Ideas, CARTOON, POP - LARGE CORK POP 01
- Sound Ideas, CARTOON, POP - LITTLE POP
- Sound Ideas, CARTOON, SCREAM - SHORT TERROR SCREAM, HORROR
- Sound Ideas, CARTOON, SLIDE - SHORT VIOLIN SLIDE UP
- Sound Ideas, CARTOON, SQUAWK - BIG HORN SQUAWK
- Sound Ideas, CARTOON, SQUEAK - SEVERAL RUBBER SQUEAKS, STRETCH
- Sound Ideas, CARTOON, SWISH - SINGLE FAST WHIPPY SWISH BY
- Sound Ideas, CARTOON, TWANG - WOBBLING TWANG
- Sound Ideas, CARTOON, WHISTLE - FLUTTER WHISTLE: LONG SPINNING WHISTLE
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST SLIDE UP, ZIP 01
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST SLIDE UP, ZIP 02
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: FAST ZIP DOWN
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: QUICK ZIP DOWN
- Sound Ideas, CARTOON, WHISTLE - SLIDE WHISTLE: SEVERAL QUICK ZIPS UP 01 (4th zip)
- Sound Ideas, CARTOON, WHIZZ - FAST WHIZZ BY (high and normal pitches)
- Sound Ideas, GUN, RICOCHET - SHORT THIN RICOCHET, BULLET 02
- Sound Ideas, HIT, CARTOON - COCONUT HIT 02
- Sound Ideas, HIT, CARTOON - HOLLOW METALLIC HEAD KONK AND HORN
- Sound Ideas, HIT, CARTOON - KABONG
- Sound Ideas, HUMAN, KISS - LONG KISS
- Sound Ideas, HUMAN, SCREAM - SCREAM: FEMALE, HORROR 03
- Sound Ideas, HUMAN, WHISTLE - CALLING WHISTLE 01
- Sound Ideas, PICK, CARTOON - FIDDLE PICK AND RISE, MEDIUM 01
- Sound Ideas, PING, CARTOON - SMALL STRING PLUCK 01
- Sound Ideas, PLINK, CARTOON - SOUR PLINK (Heard when a crab pinches Brainy Smurf's nose.)
- Sound Ideas, POP, CARTOON - HOYT'S POP
- Sound Ideas, RICOCHET - SHOT AND WHINE
- Sound Ideas, RUN, CARTOON - BLOP GALLOP, LONG/SHORT
- Sound Ideas, RUN, CARTOON - FAST RUN, LONG/SHORT (Heard mixed with Sound Ideas, TIP TOE, CARTOON - XYLOPHONE TIP TOE, LONG/MEDIUM/SHORT.)
- Sound Ideas, SKID, CARTOON - BENT SKID
- Sound Ideas, SPACE, JET PACK - JET BACK PACK, APPROACH LEFT, SHUT DOWN, ROCKET/ROCKET, SCI FI - JET PACK: APPROACH, LAND, SHUT DOWN 01
- Sound Ideas, SQUEAK, CARTOON - SINGLE RUB SQUEAK 01
- Sound Ideas, SQUEAK, CARTOON - SINGLE RUB SQUEAK 02
- Sound Ideas, SQUISH, CARTOON - LITTLE SQUISH
- Sound Ideas, STRETCH, CARTOON - RUBBER STRETCH, LONG
- Sound Ideas, TIP TOE, CARTOON - XYLOPHONE TIP TOE, LONG/MEDIUM/SHORT (Heard mixed with RUN, CARTOON - FAST RUN, LONG/SHORT.)
- Sound Ideas, TWANG, CARTOON - HOYT'S BOW TWANG
- Sound Ideas, TWANG, CARTOON - METALLIC RULER VIBRATE
- Sound Ideas, TWANG, CARTOON - RUBBER BAND TWANG, SINGLE 01
- Sound Ideas, WHINE, CARTOON - SHELL SCREAMING WHINE DOWN (Heard quadrice:)
- When Brainy Smurf falls onto Hefty Smurf's arms after his surfboard malfunctions preceded by CARTOON, WHIZZ - FAST WHIZZ BY.
- When a mouse drops an ice ball on Gargamel.
- When the bridge falls while being damaged.
- When Gargamel, Arazel and Monty fly from Gargamel's lair.
- WB CARTOON, BOING - WACKY JEWS HARP BOING
- WB CARTOON, HIT - BIG HEAD HIT
- WB CARTOON, THUNK - SMALL THUNK
- Wilhelm Scream