The show primarily uses Series 6000 sound effects, alongside minor selections from Hanna-Barbera, Cartoon Trax Volume 2, Series 4000, Premiere Edition Volume 1 and one Cartoon Trax Volume 1, Series 1000. Warner Bros., The International and Authentic Sound Effects Volume 5 sound effect.
- Elektra Records, Blizzard/Sound Ideas, WIND - SIBERIAN WIND, WEATHER (Heard once in "Beyond Your Wildest Dreams" and "Mathstronauts!".)
- Elektra Records, Seagulls
- Fiddle From Blue's Clues Trill (Heard once in "Little Red Riding Blue".)
- Hollywoodedge, Bell Tree Slow Glis CRT2025702 (Heard in:)
- "Snacktime Playdate" (4th gliss up; used twice in for Moona's Wand.)
- "Fred's Birthday" (4th gliss up; used once in a low volume for Moona's Wand in the intro.)
- "Holiday Wishes" (4th gliss up; used once in for Moona's Wand in the intro.)
- "It's Hug Day" (4th gliss up; used once in a low volume for Moona's Wand in the intro.)
- "Beyond Your Wildest Dreams!" (4th gliss up; used once in a low volume for Moona's Wand in the intro and in a normal volume in the episode.)
- "Alphabet Power" (4th gliss up; used once in a low volume for Moona's Wand in the intro.)
- "Meet Blue's Baby Brother" (4th gliss up; used once for Moona's Wand in the intro when the doors open, mixed with Sound Ideas, DOOR, WOOD - OPEN 02 and heard thrice in the episode when Joe pulls out his golden notebook.)
- "Blue's Farm Playdate" (4th gliss up; used once in for Moona's Wand in the Season 2 intro.)
- "Shape Detectives" (4th gliss up; used once in for Moona's Wand in the Season 2 intro.)
- "Masterpiece Museum" (4th gliss up; used once in for Moona's Wand in the Season 2 intro.)
- "Sprinkles' Sleepover" (4th gliss up; used once in for Moona's Wand in the Season 2 intro and in the end.)
- "World Travelers" (4th gliss up; used once in for Moona's Wand in the Season 2 intro.)
- "Mathstronauts!" (4th gliss up; used once in for Moona's Wand in the Season 2 intro.)
- "Away Great Playdate" (4th gliss up; used once in a normal pitch for Moona's Wand in the intro and heard thrice in a high and a higher pitch in the episode.)
- "Little Red Riding Blue" (4th gliss up; used once in a normal pitch for Moona's Wand in the Season 2 intro and heard quadrice in a highest pitch in the episode.)
- "Knights of the Snack Table" (4th gliss up; used once in for Moona's Wand in the Season 2 intro.)
- "Music Stars" (4th gliss up; used once in a normal pitch for Moona's Wand in the Season 2 intro and heard once in a very low, lower, low and a lowest pitch in the episode.)
- Hollywoodedge, Bird Duck Quacks Clos PE020501 (Heard twice in "Alphabet Power".)
- Hollywoodedge, Country Night Ambien PE012301 (Heard once in "Alphabet Power" and quadrice in a normal volume and once in a low to normal volume in "Sprinkles' Sleepover".)
- Hollywoodedge, Timpani Drum Boing CRT2010502 (Slowed down; heard once in "Meet Blue's Baby Brother".)
- Hollywoodedge, Yng Girl Giggl Playfu PE674101 (Heard in its logo in 2004 airings.)
- Kids Cheering and Applause from Blue's Clues (Heard twice in "Fred's Birthday".)
- Sound Ideas, AIRPLANE, BIPLANE - STEARMAN: FROM COCKPIT, START, IDLE (Heard once in a low volume and thrice in a normal volume in "World Travelers".)
- Sound Ideas, AUTO, ANTIQUE - MODEL A: START AND PULL AWAY (Heard once in a low volume and twice in a normal volume in "Meet Blue's Baby Brother".)
- Sound Ideas, AUTO, OLD. CUTLASS - EXTERNAL: HORN, DOUBLE BLAST, DISTANT, OLDSMOBILE (Heard once in "Meet Blue's Baby Brother" and "Away Great Playdate".)
- Sound Ideas, BIRD, DUCKS - AMBIENCE ON POND (Heard once in "Away Great Playdate".)
- Sound Ideas, BIRD, DUCKS - MALLARD: TWO DUCKS CALLING, ANIMAL (Heard 19 times in a normal volume and once in a low volume in "Sprinkles' Sleepover" and once in a normal volume in "Away Great Playdate".)
- Sound Ideas, BIRD, OWL - CALLING, ANIMAL 02 (Heard once in "Sprinkles' Sleepover".)
- Sound Ideas, BIRD, SPARROW - WHITE THROATED SPARROW CHIRPING, ANIMAL (Heard once in a high pitch in "Beyond Your Wildest Dreams".)
- Sound Ideas, BIRDS, JUNGLE - AFTERNOON JUNGLE BIRDS CALLING, ANIMAL (Heard once in "Beyond Your Wildest Dreams".)
- Sound Ideas, BOING, CARTOON - FLAT JEWS HARP BOING (Heard once in "Blue's Farm Playdate", mixed with CRASH, METAL - SMALL METAL PIECES DROPPING.)
- Sound Ideas, BOING, CARTOON - GOOD SPRONG 01 (Heard whenever Polka Dots pops up in a Peekaboo door.)
- Sound Ideas, BOING, CARTOON - GOOD SPRONG 02 (Heard in:)
- "Snacktime Playdate" (Cut-off; 5 times, 6 times in a normal pitch, twice in a very low and a high pitch and once in a higher pitch.)
- "Fred's Birthday" (Cut-off; once)
- "Holiday Wishes" (Cut-off; once)
- "It's Hug Day" (Cut-off; twice)
- "Beyond Your Wildest Dreams" (Cut-off; once)
- Sound Ideas, BOING, CARTOON - RIPPLE BOING (Heard 7 times in "Fred's Birthday".)
- Sound Ideas, BOOK - CLOSE (Heard once in "Snacktime Playdate", "Fred's Birthday", "Beyond Your Wildest Dreams" and "Alphabet Power".)
- Sound Ideas, BOOK - FLIP THROUGH (Heard quadrice in "It's Hug Day".)
- Sound Ideas, BOOK - OPEN (Heard once in "Snacktime Playdate", "Fred's Birthday" and "Little Red Riding Blue".)
- Sound Ideas, BOOK - TURN THREE PAGES (Heard in:)
- "Snacktime Playdate" (1st page turn; quadrice)
- "Fred's Birthday"
- "Masterpiece Museum" (2nd page turn; twice)
- "Sprinkles' Sleepover" (2nd page turn)
- Sound Ideas, BOX, CARDBOARD - CARDBOARD BOX FALLING ON GROUND 01 (Heard once in a normal and a low volume in "Fred's Birthday".)
- Sound Ideas, CAMERA - 35 MM SLR WITH AUTO WINDER: SINGLE SHOT (Heard in the Season 2 intro.)
- Sound Ideas, CARTOON, BOING - JEW'S HARP BOING, SHORT 01 (Cut-short; heard once in a high pitch in "World Travelers".)
- Sound Ideas, CARTOON, POP - SMALL POP 01 (Heard quadrice in "Beyond Your Wildest Dreams".)
- Sound Ideas, CARTOON, POP - SMALL POP 02 (Heard once in "Beyond Your Wildest Dreams".)
- Sound Ideas, CARTOON, POP - SMALL VOCAL POP 01
- Sound Ideas, CHIMES - MAGIC CHIMES RINGING
- Sound Ideas, COUNTRY, DAY - BIRDS, CRICKETS, STEADY BREEZE, AMBIENCE (Heard once in the Season 2 intro, mixed with ORCHESTRA - WARMING UP IN CONCERT HALL, MUSIC. It is also used in a low volume in "Meet Blue's Baby Brother" and in a normal volume in "Mathstronauts" and "Away Great Playdate".)
- Sound Ideas, CRASH, METAL - SMALL METAL PIECES DROPPING (Heard once in "Blue's Farm Playdate", mixed with BOING, CARTOON - FLAT JEWS HARP BOING.)
- Sound Ideas, DOOR, BELL - SINGLE RING (Heard once in "Holiday Wishes", "Away Great Playdate" and twice in "Alphabet Power!".)
- Sound Ideas, DOOR, CABINET - CLOSE 03 (Heard in:)
- "Shape Detectives" (thrice)
- "Masterpiece Museum" (once in low volume and thrice in a normal volume)
- "Mathstronauts!" (thrice)
- "Music Stars"
- Sound Ideas, DOOR, LOCK - TURN AND LOCK 01
- Sound Ideas, DOOR, LOCK - TURN AND LOCK 03
- Sound Ideas, DOOR, LOCK - TURN AND LOCK 05
- Sound Ideas, DOOR, WOOD - CLOSE 02 (Heard once in the end of "Meet Blue's Baby Brother", "Blue's Farm Playdate", "Shape Detectives", "World Travelers", "Mathstronauts!", "Away Great Playdate", "Little Red Riding Blue", "Knights of the Snack Table" and "Music Stars".)
- Sound Ideas, DOOR, WOOD - OPEN 02 (Heard thrice in "Meet Blue's Baby Brother" once in "Masterpiece Museum" and twice in "Little Red Riding Blue".)
- Sound Ideas, DOOR, WOOD - SQUEAKY OPEN (Hinge only, cut-off; heard 20 times in "Meet Blue's Baby Brother" and once in "Shape Detectives". Heard normally in "Blue's Farm Playdate".)
- Sound Ideas, ELECTRONIC - MAGICAL ZING 01 (Heard in "Beyond Your Wildest Dreams".)
- Sound Ideas, ELECTRONIC - MAGICAL ZING 03 (Heard once in a normal pitch in "Knights of the Snack Table" and twice in a low pitch, 6 times in a normal pitch and once in a high pitch in "Blue's Farm Playdate".)
- Sound Ideas, FLY - SINGLE FLY BUZZING, ANIMAL, INSECT (H-B) (Heard in "Beyond Your Wildest Dreams".)
- Sound Ideas, FROG, BULLFROG - CROAKING, ANIMAL, AMPHIBIAN 02 (Heard once in "Beyond Your Wildest Dreams".)
- Sound Ideas, HATCH, SUBMARINE - METAL HATCH ON SUBMARINE: OPEN 01 (1st half; heard twice in a low volume in "Meet Blue's Baby Brother".)
- Sound Ideas, HELICOPTER - BELL 206: EXTERNAL: APPROACH, PASS BY, FAST (Heard once in "Away Great Playdate".)
- Sound Ideas, HORN, BICYCLE - BULB HORN: SINGLE HONK, TOY (Heard once in "Beyond Your Wildest Dreams".)
- Sound Ideas, HORSE - INTERIOR: WHINNY, ANIMAL 01 (Heard once in "Knights of the Snack Table".)
- Sound Ideas, HORSE - INTERIOR: WHINNY, ANIMAL 02 (Heard quadrice in "Knights of the Snack Table".)
- Sound Ideas, LEAVES - LEAVES: RUSTLING 02 (Heard twice in "Blue's Farm Playdate" and "World Travelers.)
- Sound Ideas, MAIL BOX, METAL - OPEN LID, LETTER, POST (Heard twice in "Meet Blue's Baby Brother".)
- Sound Ideas, MOTOR, SUBMARINE - SUBMARINE TURBO START MOTOR, WIND UP, INDUSTRY (Heard once in "Mathstronauts!", mixed with TAPE RECORDER - PORTABLE CASSETTE: START BUTTON.)
- Sound Ideas, NOISEMAKER - HORN: TOOT, PARTY, TOY (Heard often in "Meet Blue's Baby Brother".)
- Sound Ideas, NOISEMAKER - PAPER BLOWOUT WITH HORN, PARTY (Heard thrice in "Fred's Birthday" and twice in "Meet Blue's Baby Brother".)
- Sound Ideas, ORCHESTRA - WARMING UP IN CONCERT HALL, MUSIC (Heard once in the intro of "Meet Blue's Baby Brother", mixed with ORCHESTRA BELLS - DREAMING, SHORT, MUSIC, PERCUSSION. It is also heard in the season 2 intro.)
- Sound Ideas, ORCHESTRA BELLS - DREAMING, SHORT, MUSIC, PERCUSSION
- Sound Ideas, ORCHESTRA BELLS - GLISS, DOWN, MUSIC, PERCUSSION (Heard once in "Meet Blue's Baby Brother".)
- Sound Ideas, ORCHESTRA BELLS - GLISS, UP, MUSIC, PERCUSSION (Heard in:)
- "Fred's Birthday" (once)
- "Alphabet Power" (once)
- "Meet Blue's Baby Brother" (slower tempo and normal edits)
- "Little Red Riding Blue" (twice)
- Sound Ideas, ORCHESTRA BELLS - TRILL, MUSIC, PERCUSSION 02 (Heard once in "Meet Blue's Baby Brother".)
- Sound Ideas, SCI FI - LEGION OF DOOM BACKGROUND (Heard once in "Mathstronauts!".)
- Sound Ideas, SHAKER, SALT - SHAKE SALT SHAKER (Used for Mr. Salt & Mrs. Pepper's movement in "Meet Blue's Baby Brother".)
- Sound Ideas, SHOVEL, DIRT - SHOVELING GRITTY DIRT, GARDENING, DIGGING (Heard once in "Blue's Farm Playdate".)
- Sound Ideas, SPLAT, CARTOON - SLOPPY MUD PLOPS (Heard once in "Snacktime Playdate", "Little Red Riding Blue" and "Knights of the Snack Table".)
- Sound Ideas, SQUEAK, METAL - TURNING
- Sound Ideas, TAPE RECORDER - PORTABLE CASSETTE: INSERT CASSETTE (Heard once in "Snacktime Playdate".)
- Sound Ideas, TAPE RECORDER - PORTABLE CASSETTE: REMOVE CASSETTE (Heard twice in "Snacktime Playdate".)
- Sound Ideas, TAPE RECORDER - PORTABLE CASSETTE: START BUTTON (Heard in:)
- "Snacktime Playdate" (low volume)
- "Mathstronauts!" (mixed with MOTOR, SUBMARINE - SUBMARINE TURBO START MOTOR, WIND UP, INDUSTRY)
- "Music Stars"
- Sound Ideas, TAPE RECORDER - PORTABLE CASSETTE: STOP BUTTON (Heard once in "Beyond Your Wildest Dreams".)
- Sound Ideas, TELEPHONE, DOMESTIC - OLD DIAL PHONE: BELL RINGING (Heard thrice in "Shape Detectives".)
- Sound Ideas, TELEPHONE, DOMESTIC - OLD DIAL PHONE: HANG UP RECEIVER (Heard once in "Little Red Riding Blue".)
- Sound Ideas, WATER, LAKE - WATER LAPPING ON ROCK SHORE, LIGHT, WAVES (Heard in a normal volume in "Beyond Your Wildest Dreams" and in a low volume in "Meet Blue's Baby Brother".)
- Sound Ideas, WATER, SPLASH - EARS LIGHT SPLASH, CARTOON 02 (Heard once in "Away Great Playdate".)
- Sound Ideas, XYLOPHONE - GLISS UP, SHORT, MUSIC, PERCUSSION (Heard twice in "Snacktime Playdate" and once in "Fred's Birthday".)
- Sound Ideas, XYLOPHONE - SNEAKY, MUSIC, PERCUSSION 01 (Heard once in "Snacktime Playdate" and "Fred's Birthday".)
- Sound Ideas, XYLOPHONE - SNEAKY, MUSIC, PERCUSSION 02 (Heard in:)
- "Fred's Birthday" (low and normal volumes)
- "Meet Blue's Baby Brother"
- "Blue's Farm Playdate"
- "World Travelers" (last part only)